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KMID : 0380020140290020081
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 2 p.81 ~ p.86
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Improved Cell Viability of Lactobacillus crispatus KLB46 by Stress Adaptation
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Kwak Dae-Yung
Kang Chang-Ho
Jeon Han-Eul
So Jae-Seong
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Abstract
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Lactobacilli, the dominant species of microorganisms in the vaginal flora of healthy women, play important roles to prevent bacterial vaginosis and other sexually transmitted diseases. In this study, we carried out studies on stress adaptation prior to various stress treatment. We found that heat or salt adapted KLB46 showed higher cell viability than non adapted upon heat stress at 60°C for 20 min. When chloramphenicol was added during the adaptation process, heat tolerance was abolished. This result suggested that de novo protein synthesis was essential during adaptation.
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KEYWORD
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Lactobacillus crispatus KLB46, adaptation, stress response, cell viability
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